Journal: Scientific Reports

Article Title: Unraveling Ginsenoside Rg1’s osteoprotective pathways in zebrafish models of glucocorticoid induced osteoporosis via transcriptomics

doi: 10.1038/s41598-025-15284-2

Figure Lengend Snippet: Effects of PN, ED, and different concentrations of Rg1 on calcein staining area and IOD in zebrafish larvae. ( A ) Fluorescence imaging of calcein staining in zebrafish larvae, showing the 1–3 vertebral segments used for quantification. ( B ) Quantification of the calcein staining area in the 1–3 vertebral segments of zebrafish larvae across different groups. ( C ) Fluorescence IOD of the 1–3 vertebral segments in zebrafish larvae across different groups. Con: Control group; PN: 25 μM Methylprednisolone; ED: 15 μg/mL Etidronate disodium + 25 μM PN; R 25 : 25 μM Rg1 + 25 μM PN; R 50 : 50 μM Rg1 + 25 μM PN; R 100 : 100 μM Rg1 + 25 μM PN. Scale bar: 100 μm. Different letters above the bars indicate statistically significant differences ( P < 0.05).

Article Snippet: Ginsenoside Rg1 (CAS No. 22427–39–0, 99% purity) was purchased from TargetMol (TargetMol, USA).

Techniques: Staining, Fluorescence, Imaging, Control

Journal: Scientific Reports

Article Title: Unraveling Ginsenoside Rg1’s osteoprotective pathways in zebrafish models of glucocorticoid induced osteoporosis via transcriptomics

doi: 10.1038/s41598-025-15284-2

Figure Lengend Snippet: Effects of PN, ED, and different concentrations of Rg1 on ARS area and IOD in zebrafish larvae skulls. ( A ) Images of the zebrafish larvae skulls stained with alizarin red at 8 dpf. ( B ) Quantification of the ARS area in the zebrafish larvae skulls across different groups. ( C ) ARS IOD of the zebrafish larvae skulls in different groups. Scale bar: 200 μm. Different letters above the bars indicate statistically significant differences ( P < 0.05).

Article Snippet: Ginsenoside Rg1 (CAS No. 22427–39–0, 99% purity) was purchased from TargetMol (TargetMol, USA).

Techniques: Staining

Journal: Scientific Reports

Article Title: Unraveling Ginsenoside Rg1’s osteoprotective pathways in zebrafish models of glucocorticoid induced osteoporosis via transcriptomics

doi: 10.1038/s41598-025-15284-2

Figure Lengend Snippet: ARS of different parts of zebrafish larvae skulls in different groups (n > 9) and the protective effects of Rg1 on bone-related gene expressions in GIOP zebrafish at 8 dpf. ( A ) Schematic representation of various parts of the cranium in zebrafish larvae at 8 dpf. The mineralisation IOD of ( B ) mc, ( C ) cl, ( D ) hm, ( E ) ps, and ( F ) ch. Relative mRNA expression of ( G ) bglap , ( H ) op1 , ( I ) rankl , and ( J ) traf . Mk: Meckel’s cartilage; hm: hyoid mandibular arch; op: operculum; cl: cleithrum; ps: parasphenoid bone; oc: occipital bone; nc: notochord; ch: ceratohyal; br: branchial ray. Scale bar: 200 μm. Different letters above the bars indicate statistically significant differences ( P < 0.05).

Article Snippet: Ginsenoside Rg1 (CAS No. 22427–39–0, 99% purity) was purchased from TargetMol (TargetMol, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Unraveling Ginsenoside Rg1’s osteoprotective pathways in zebrafish models of glucocorticoid induced osteoporosis via transcriptomics

doi: 10.1038/s41598-025-15284-2

Figure Lengend Snippet: Profile of changes in Rg1-induced gene expression. ( A ) Venn diagram showing the reversal of expression in 383 DEGs. ( B ) Expression heatmap showing the reversed expression of the 383 DEGs across the control, 25-μM PN, and 50-μM Rg1 groups. ( C ) GO enrichment scatterplots of the 383 reversed genes. ( D ) KEGG enrichment analysis scatterplots of the 383 reversed genes. ( E ) PPIN of the 242 upregulated reversed genes. ( F ) PPIN of DEGs enriched in signalling pathways related to skeletal system development.

Article Snippet: Ginsenoside Rg1 (CAS No. 22427–39–0, 99% purity) was purchased from TargetMol (TargetMol, USA).

Techniques: Gene Expression, Expressing, Control